Synthesis and Characterizations of Hydroxyapatite from Bovine Bone Using Alkaline Hydrolysis Method

Synthesis and Characterizations of Hydroxyapatite from Bovine Bone Using Alkaline Hydrolysis Method

• April 2018

• DOI: 
• 10.23960/ins.v3i1.124

• Nuning Aisah
• Muslim Efendi Harahap
• Dwi Budianto
• Dwi gustiono Riban

Abstract

In this research, the synthesis of hydroxyapatite compound (HAp) has been done by extracting technique from cow cortex thigh bones to eliminate organic compounds contained in bone so that it can be used as raw material of bone graft. The method used is alkaline hydrolysis method of soaking bone in NaOH solution with variation of concentration (1M, 2M, 3M) and immersion time (30 minutes, 60 minutes, 90 minutes). The extracted HAp powder was then analyzed using FTIR, XRD and SEM. The results showed that a hydroxyapatite powder with a soaking time of 30 minutes and a 2M and 3M NaOH concentration did not contain organic elements. The XRD peak pattern shows the phase formed is a hydroxyapatite with a hexagonal structure having an average crystal size of 10-12 nm.

Production and Characterization of Scaffold made of Hydroxyapatite and Pectin of Green Cincau Leaf (Premna Oblongifolia Merr)

Production and Characterization of Scaffold made of Hydroxyapatite and Pectin of Green Cincau Leaf (Premna Oblongifolia Merr)

• June 2019

• DOI: 
• 10.23960/jesr.v1i1.4

• S Habibie
• Y Tristiyanti
• Dwi gustiono Riban
• Mochammad Dachyar Effendi
• Abstract
  Scaffold is a 3-dimensional matrix created as a new bone cell growth medium madefrom natural polymers and bioceramics. The extracted pectin from green Cincau leaves(Premna oblongifolia Merr) and hydroxyapatite (HA) are used in the manufacture ofscaffolds. Pectin was extracted using citric acid with variation concentration of 0, 0.1,0.2 and 0.3% (w / v). The 3% (w / w) HA-pectin mixture, dried freeze using a freezedryer. The characterization of extracted pectin and HA-pectin scaffold was thenperformed. The results showed that pectin of green Cincau leaves had low methoxylcontent, which was 1.364 to 5.022%. The resulting scaffold has a pore size rangingfrom 8.25 to 115 µm while the scaffold resistance to the load, ie 0.03 to 0.15 MPa. Thescaffold porosity that has been made is 15.33 to 40.97% while the density is 0.69 to1.02 g/cm3Keywords: Green Cincau Leaf, Hydroxyapatite, Pectin, Scaffold