Comparison on mechanical properties of single layered and bilayered chitosan-gelatin coated porous hydroxyapatite scaffold prepared through freeze drying method
M D Effendi, D Gustiono, Lukmana, D Ayu and F Kurniawati
Published under licence by IOP Publishing Ltd
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Abstract
Biopolymer coated porous hydroxyapatite (HA) scaffolds
were prepared for tissue engineering trough freeze drying method and
impregnation. in this study, to mimic the mineral and organic component
of natural bone, synthetic hydroxapatite (HA) scaffolds coated by
polymer were prepared. Highly porous Hap scaffolds, fabricated by
synthetic HA impregnation method on polyurethane foam, were coated with
polymer coating solution, consisting of chitosan, Gelatin, and bilayered
chitosan-gelatin prepared by aging and impregnating technique. For the
purpose of comparison, The bare scaffolds without polymer coating layer
were investigated. The Bare scaffolds were highly porous and
interconnected with a pore size of around 150 µm–714 µm, has porosity at
around 67,7% -85,7%, and has mechanical strength at around 0.06 Mpa -
0.071 Mpa, which is suitable for osteoblast cell Proliferation. Chitosan
coated porous HA scaffold and gelatin coated porous HA scaffold had
mechanical strength at around 0.81-0.85 Mpa, and 1.32-1.34 Mpa,
respectively, with weight ratio of biopolymer and Hap was around
18%-22%. To compare these results, the coating on the bare scaffold with
gelatin and chitosan had been conducted. Based on the result of FTIR,
it could be concluded that coating procedure applied on porous hydroxy
apatite (HA) coated by gelatin, chitosan coated HA scaffold, and
bilayered Gelatin-chitosan coated porous HA scaffold, confirming that
for allsampleshad no significant chemical effect on the coating
structure. The compressive strength of bilayered Gelatin-chitosan coated
HA scaffold had middle values between the rest, at around 1,06-1.2 Mpa
for the samples at the same weight ratio of biopolymer: HA (around 18% -
22%). These results also confirming that coating by gelatin on porous
hydroxyapatite was highest compresive strength and can be applied to
improve mechanical properties of porous hydroxyapatite bare scaffold
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