Fabrikasi granul HA enkapsulasi

Synthesis and Characterizations of Hydroxyapatite from Bovine Bone Using Alkaline Hydrolysis Method

Synthesis and Characterizations of Hydroxyapatite from Bovine Bone Using Alkaline Hydrolysis Method

• April 2018

• DOI: 
• 10.23960/ins.v3i1.124

• Nuning Aisah
• Muslim Efendi Harahap
• Dwi Budianto
• Dwi gustiono Riban

Abstract

In this research, the synthesis of hydroxyapatite compound (HAp) has been done by extracting technique from cow cortex thigh bones to eliminate organic compounds contained in bone so that it can be used as raw material of bone graft. The method used is alkaline hydrolysis method of soaking bone in NaOH solution with variation of concentration (1M, 2M, 3M) and immersion time (30 minutes, 60 minutes, 90 minutes). The extracted HAp powder was then analyzed using FTIR, XRD and SEM. The results showed that a hydroxyapatite powder with a soaking time of 30 minutes and a 2M and 3M NaOH concentration did not contain organic elements. The XRD peak pattern shows the phase formed is a hydroxyapatite with a hexagonal structure having an average crystal size of 10-12 nm.

Production and Characterization of Scaffold made of Hydroxyapatite and Pectin of Green Cincau Leaf (Premna Oblongifolia Merr)

Production and Characterization of Scaffold made of Hydroxyapatite and Pectin of Green Cincau Leaf (Premna Oblongifolia Merr)

• June 2019

• DOI: 
• 10.23960/jesr.v1i1.4

• S Habibie
• Y Tristiyanti
• Dwi gustiono Riban
• Mochammad Dachyar Effendi
• Abstract
  Scaffold is a 3-dimensional matrix created as a new bone cell growth medium madefrom natural polymers and bioceramics. The extracted pectin from green Cincau leaves(Premna oblongifolia Merr) and hydroxyapatite (HA) are used in the manufacture ofscaffolds. Pectin was extracted using citric acid with variation concentration of 0, 0.1,0.2 and 0.3% (w / v). The 3% (w / w) HA-pectin mixture, dried freeze using a freezedryer. The characterization of extracted pectin and HA-pectin scaffold was thenperformed. The results showed that pectin of green Cincau leaves had low methoxylcontent, which was 1.364 to 5.022%. The resulting scaffold has a pore size rangingfrom 8.25 to 115 µm while the scaffold resistance to the load, ie 0.03 to 0.15 MPa. Thescaffold porosity that has been made is 15.33 to 40.97% while the density is 0.69 to1.02 g/cm3Keywords: Green Cincau Leaf, Hydroxyapatite, Pectin, Scaffold

Monitoring dan evaluasi (Monev) program PPTI Bonegraft

Tahun anggarran 2019, tim biomat keramik-komposit memiliki dua kegiatan yang didanai oleh Kemenristikdikti, melalui program Pengembangan Teknologi di Indutri (PPTI). judul-judul dari kegiatan tersebut adalah :

1. Pengembangan Teknologi Fabrikasi Bone Filler  Hydroxyapatite dari Batu gamping lokal Untuk Aplikasi Orthopedik dan Bedah Gigi Dalam Mendukung Kemandirian Produk Alat Kesehatan Nasional

late post : INTERNATIONAL SEMINAR ON NATURAL PRODUCT MEDICINE 2018

INTERNATIONAL SEMINAR ON NATURAL PRODUCT MEDICINE 2018

Sekolah Farmasi - Institut Teknologi Bandung

Bandung,  4-5 Oktober 2018

DEVELOPMENT OF BONE SCAFFOLD BASED ON HYDROXYAPATITE - CINCAU PECTIN BIOCOMPOSITE

Pectin extract results from green Cincau leaf (Premna oblongifolia Merr) using citric acid showed that the increase of citric acid concentration could increase the amount of yields and metoxyl content, but the equivalent weight decreased with the increase of citric acid concentration. While the degree of esterification is relatively low despite the increased concentration of citric acid, it is also supported by the results of observation with FTIR.

The observations by FTIR of the resulting pectin show there are carboxyl groups and metal groups of the ester and ether groups, as well as the C-O and C-OH bonds of the pyranose chain.  The result of morphological analysis and compressive strength of scaffold made by SEM observation, it still could not reach optimum and fragile pore size. The result of porosity analysis and the density of scaffold made has fulfilled the porosity range and sponge bone density and can experience degradation. The result also shows the difference of pore size between the surface part and the center of the scaffold where the surface part has a larger pore than the center of the scaffold.

Between HA and pectin, there appears ionic bonding and hydrogen bonding, however the HA-pectin scaffold has low strength. The results of degradation testing showed that there was an increase in degradation with increasing days of observation and reached the maximum degradation on day 14, after which degradation decreased.